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The substantially reduce expression stage acquired from the microarray knowledge for this gene could be partly discussed by the properly-known underestimation of expression ratios by microarrays in contrast with RT-PCR [29]. The expression of the CYP4G61 gene in the unique unselected subject pressure TV8 was also examined by RT-PCR (Desk 3)

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Genuine-time quantitative PCR was utilised to validate the microarray benefits by analyzing the expression...