Product Name :
Anti-HA tag, Rabbit antibody(Biotin)
Applications:
WB,ICC/IF,ELISA,IP
Reactivity :
HA tag
Conjugate:
Biotin
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-HA tag, Rabbit antibody(Biotin) is designed for detecting HA tag fusion proteins specifically. Anti-HA tag, Rabbit antibody(Biotin) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to HA tag coupled to Biotin, and Anti-HA tag, Rabbit antibody(Biotin) detects the HA tag selectively, no reactivity with other proteins. | | Immunogen: HA tag fused KLH | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc | Conjugate: Biotin | Specificity: HA tag(YPYDVPDYA) | Cross-Reactivity: Highly selective for HA tag sequence | Affinity: Dissociation constant KD of 0.1nM | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Buffer: 10mM PBS (pH 7.{{2769152-95-4} medchemexpress|{2769152-95-4} Purity & Documentation|{2769152-95-4} In Vivo|{2769152-95-4} custom synthesis} 5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300 | Storage: Store at –20 °C(Avoid freeze / thaw cycles)Background:The HA tag is widely used for detecting, manipulating or purifying proteins.{{84573-16-0} medchemexpress|{84573-16-0} Biological Activity|{84573-16-0} References|{84573-16-0} custom synthesis} This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, HA tag is unlikely to affect the tagged protein’s biochemical properties. HA tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.PMID:30252250
Description2 :
Anti-HA tag, Rabbit antibody(Biotin) is designed for detecting HA tag fusion proteins specifically. Anti-HA tag, Rabbit antibody(Biotin) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to HA tag coupled to Biotin, and Anti-HA tag, Rabbit antibody(Biotin) detects the HA tag selectively, no reactivity with other proteins.
Immunogen:
HA tag fused KLH
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc
Purity :
Recombinant Expression and Affinity purified
Buffer :
10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage :
Function:
WB: 1:5,000-1:20000 ELISA: 1:5,000-1:20000IP: 1-2ug/sampleDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
